Indazole-Type Alkaloids from Nigella sativa Seeds Exhibit Antihyperglycemic Effects via AMPK Activation in Vitro
نویسندگان
چکیده
Six rare naturally occurring indazole-type alkaloids including two new compounds, 17-O-(β-d-glucopyranosyl)-4-O-methylnigellidine (1) and nigelanoid (2), and four known compounds (3-6) were isolated from a defatted extract of Nigella sativa (black cumin) seeds. 17-O-(β-d-Glucopyranosyl)-4-O-methylnigellidine (1) increased glucose consumption by liver hepatocytes (HepG2 cells) through activation of AMP-activated protein kinase (AMPK). Also, this is the first report of compounds 4 and 6 from a natural source.
منابع مشابه
An Efficient DNA Isolation Method from Nigella sativa L. (Ranunculaceae) Seeds for RAPD and ISSR Analysis
Nigella sativa L. (Ranunculaceae) seeds (black cumin) that contain high oil, terpene, protein, alkaloids are an economically important for medicine and food industry. Lack of an efficient DNA isolation procedure is a limiting factor for any molecular studies of these plant. We have used a genomic DNA isolation protocol for Nigella sativa seeds based on a hexadecyltrimethylammonium bromide (CTAB...
متن کاملDistribution of primary and specialized metabolites in Nigella sativa seeds, a spice with vast traditional and historical uses.
Black cumin (Nigella sativa L., Ranunculaceae) is an annual herb commonly used in the Middle East, India and nowadays gaining worldwide acceptance. Historical and traditional uses are extensively documented in ancient texts and historical documents. Black cumin seeds and oil are commonly used as a traditional tonic and remedy for many ailments as well as in confectionery and bakery. Little is k...
متن کاملEvaluation of cytotoxic and apoptotic effects of linoleic acid from Nigella sativa seeds on human ovary carcinoma cell line
Background and objectives: Black cumin is a herbaceous plant with scientific name Nigella sativa, that contains compounds such as fixed oils, proteins, alkaloids, saponins and essential oils. It has numerous health benefits including antioxidant and anti-inflammatory properties. In the current study, we have evaluated the cytotoxic effects of compounds isolated from th...
متن کاملThymoquinone, the active ingredient of Nigella sativa seeds, enhances survival and activity of antigen-specific CD8-positive T cells in vitro.
Recent preclinical and clinical studies provide evidence that adoptive transfer of in vitro activated T cells can results in significant antitumour responses in vivo upon acquisition of certain survival and homing properties during in vitro activation. Based on recent studies showing in vivo antioxidant effects of thymoquinone (TQ), the active ingredient of Nigella sativa seeds, this study aims...
متن کاملIn vitro and in vivo study of the antibacterial effects of Nigella sativa methanol extract in dairy cow mastitis
Objective: Nigella Sativa (N. Sativa) seeds were used in traditional medicine for the treatment of a variety of diseases. The seed extracts and oil of this plant have shown various pharmacological properties including antimicrobial actions. In this study, the in vitro and in vivo antibacterial effects of methanol extract of the seeds against pathogenic bacteria causing mastitis in cows have bee...
متن کامل